A next step alternative to c18 zorbax eclipse plus phenylhexyl. Comparison guide to c18 reversed phase hplc columns introduction there are so many different c18 columns to choose from, that finding the right column for a particular separation can be very time consuming and expensive. To find the columns that are most different from the one selected above, sort the column database below by f s so that the largest f s values are listed first select filters to narrow down the list of. Columns, supplies, and standards knowledgebase agilent.
The alternate selectivity of phenyl phases is often explained by the. Agilent zorbax stablebond sbphenyl, eclipse xdbphenyl and eclipse plus phenylhexyl columns. Poroshell 120 sbaq this proprietary phase provides an alternate selectivity option, and is ideal for polar compounds and. Agilents newly expanded zorbax eclipse plus lc column portfolio no other lc columns have ever produced such superior peaksfor bases, acids, and neutrals. Zorbax columns for analytical hplc zorbax eclipse xdb. The separation of the ssris have been carried out by using four different column types, including xterra ms c18, zorbax sbphenyl, alltima c18 and phenyl hypersil columns, and their. Hichrom chromatography columns and supplies hichrom limited 1 the markham centre, station road theale, reading, berks, rg7 4pe, uk tel. Zorbax hplc 25 % off zorbax hplc zorbax hplc 25 000ff hplcuhplc high definition rrhd 1. Eclipse plus phenyl hexyl is especially useful for the separation of acidic, basic, and other highly polar compounds by reversephase liquid chromatography. Hplc columns hplc column selectivity measurements of. Phenyl hexyl phenyl phase attached to c6 hexyl ligand 3, 5, 10, 10prep, 15 100 400 17. Widepore cyano selectvity for polar and nonpolar compounds. Additional zorbax lcms columns ordering information column size particle sbc18 sbc8 sbcn sbphenyl description mm size m usp l1 usp l7 usp l1 0 sbc3 usp l11 sbaq zorbax stablebond columns narrow bore 2.
Zorbax sb phenyl is compatible with water and all common organic solvents. Zorbax columns for analytical hplc zorbax eclipse xdb hplc columns. Zorbax phenyl zorbax stablebond phenyl inertsil ph phenyl inertsil ph3 phenyl metasil phenyl. Zorbax stablebond columns 80a use patented, monofunctional silanes with bulky diisobutyl sbc18 or diisopropyl sbc8, sbc3, sbphenyl, sbcn and sbaq side chain groups that sterically protect the key siloxane bond from hydrolytic attack at low ph. These are known as the stablebond family of columns. Chrom tech inc hplc columns agilent zorbax eclipse pah.
These columns provide good peak shape over a wide ph range 29 for additional method development flexibility with one family of columns. The agilent zorbax eclipse xdb columns c18, c8 and phenyl are designed. Columns are loaded to a uniform bed density using a proprietary, highpressure, slurryloading technique. Zorbax phenyl zorbax stablebondphenyl inertsil ph phenyl inertsil ph3 phenyl metasil phenyl. For consistency, the sbc8 was added to the family, but the rxc8 was not. Shodex ohpak sb803 hq hema bio 20 hema bio 40 a methacrylatebased sizeexclusion packing for water. Sbphenyl, sbc3, and sbcn are less susceptible to loss of the bonded phase by acid hydrolysis than traditional dimethylsilanes providing high column lifetime under these harsh conditions.
Zorbax sb c8 pn 883975906 zorbax eclipse xdbc8 pn 993967906. Eclipse plus phenylhexyl is especially useful for the separation of acidic, basic, and other highly polar compounds by reversephase liquid chromatography. Zorbax columns for analytical hplc zorbax eclipse xdb hplc. Analysis of polar compounds using 100% aqueous mobile. Shodex ohpak sb 803 hq hema bio 20 hema bio 40 a methacrylatebased sizeexclusion packing for water. Widepore hydrophobic selectivity for nonpolar compounds.
In some cases the recommended comparable columns may give slightly different selectivity, and may lead to improved and more reliable separation. Zorbax stablebond hplc columns krackeler scientific, inc. Sb phenyl, sb c3, and sb cn are less susceptible to loss of the bonded phase by acid hydrolysis than traditional dimethylsilanes providing high column lifetime under these harsh conditions. Zorbax stablebond phenyl welcome to lcgc knowledge park. Pdf simultaneous determination of citalopram, paroxetine. Zorbax columns provide with both short and long columns very high separation efficiencies. Suitability for highly aqueous compounds zorbax sbaq and bonusrp. View online or download agilent zorbax phenyl user manual. Stability and performance of cyano bonded phase hplc columns for reversedphase, normalphase and hilic applications richard a. Zorbax sb columns are designed to offer improved life time over traditional zorbax columns. Zorbax hplc column selection guide for biotechnology proquinorte. In this case they are the phenyl phases exemplified by. Comparison guide to c18 reversed phase hplc columns. Zorbax extendc18 columns offer high efficiency for high ph applications up to ph 11.
Two apparently similar c18 phases can give very different results. Zorbax sbc18 is a unique microparticulate c18 pack ing used for. Stability and performance of cyano bonded phase hplc. Agilent zorbax sbphenyl description agilent zorbax sbphenyl is made by chemically bonding a sterically protected phenyl stationary phase to special zorbax silica, designed to reduce or eliminate strong adsorption of basic compounds. Zorbax stablebond columns offer the highest stability for low ph applications down to ph 1 while zorbax sb aq deliver robust, reproducible separations with highly aqueous mobile phases, including 100% water. Zorbax stablebond columns offer the highest stability for low ph applications down to ph 1 while zorbax sbaq deliver robust, reproducible separations with highly aqueous mobile phases, including 100% water. For example, figure 1 compares the separation of the same. Zorbax hplc columns for lcms and high throughput hplc. Phenyl bonded silica phases offer an alternative reversedphase selectivity to alkyl bonded phases.
Agilent zorbax stablebond columns use patented, unique, nonfunctional silanes with bulky diisobutyl sb c18 or diisopropyl sb c8, sb c3, sb phenyl, sb cn, and sb aq side chain groups that sterically protect the key siloxane bond to the silica surface from hydrolytic attack at low ph. Some zorbax columns that use this type of silica are the stablebond columns, the eclipse. Zorbax rx zorbax eclipse plus zorbax extendc18 zorbax eclipse xdb zorbax bonusrp zorbax stablebond 80a pursuit xrs ultra pursuit xrs pursuit ups pursuit polaris. A next step alternative to c18 zorbax eclipse plus phenyl hexyl. Establishing a selectivity survey as part of the method development. The alternate selectivity of phenyl phases is often explained by the interactions available through the phenyl ring. At ph 68, highest column stability for all silicabased columns is obtained. Agilent 880975912 hplc column zorbax sbphenyl, 80a, 5 m. The densely covered, sterically protected, diiosopropyl phenylsilane stationary phase. Zorbax lc columns are optimized for high resolution and high throughput analysis, and feature. Product was successfully added to your shopping cart. Hplc column zorbax sbphenyl, 80a, 5 m, 4,6 x 250 mm. Agilent zorbax sbphenyl is made by chemically bonding a sterically protected phenyl stationary phase to special zorbax silica, designed to reduce or eliminate strong adsorption of basic compounds.
Stability indicating assay method for montelukast sodium. Comparison of selectivity differences among different agilent. Our wide range of phases offers excellent alternatives to zorbax, polaris, pursuit, tcc182 and hcc182. Phenyl shield rp18c 18 zorbax sbc18 desipramine amitriptyline ph 3 7 nortriptyline amitriptyline ta il in g fa ctor t f the combination of excellent particle and ligand stability as well as high chromatographic efficiencies makes xbridge columns an ideal choice for low and intermediate ph methods. Usp listing of hplc columns l packing brand name l packing brand name zorbax eclipse plus c18. Suitability for highly aqueous compounds zorbax sb aq and bonusrp. General description agilent zorbax rapid resolution high definition rrhd sb phenyl threaded columns are specially designed for use with ultra high performance liquid chromatographs uhplcs such as the agilent 1290. The densely covered, sterically protected, diiosopropyl phenylsilane stationary phase is chemically stable and gives longer column life.
Stablebond for low ph, bonusrp for low and mid ph, and extendc18 from low to high ph. Zorbax 300stablebond and stablebond reversedphase columns are. Enhance your productivity with new options for fast and ultrafast lc. Agilent zorbax rapid resolution high definition sbphenyl column controlled pore size of 80a. Agilents newly expanded zorbax eclipse plus lc column. Appendices phenomenex 385 a selection of other sorbents packing material particle shapesize. Poroshell 120 phenylhexyl usp l11 this phase provides alternative selectivity for phenyl groups, and is very similar to zorbax eclipse plus phenylhexyl for easy method transfer. Agilent zorbax sb phenyl is made by chemically bonding a sterically protected phenyl stationary phase to special zorbax silica, designed to reduce or eliminate strong adsorption of basic compounds. Whether you are performing conventional or ultrafast chromatography or analyzing complex basic compounds, agilent can be trust for the industrys highestperforming columns that deliver the fast, reproducible results you need all engineered with agilents unparalleled quality and. Ordering information for ascentis phenyl hplc columns.
Poroshell 120 phenylhexyl usp l11 this phase offers an alternative selectivity for phenyl groups, and is very similar to zorbax eclipse plus phenylhexyl for easy method transfer. Widepore intermediate hydrophobic selectvity for polar and nonpolar compounds. All product names and brands are property of their respective owners. C8, and phenyl bonded phases for selectivity optimization of most separations. Hplc columns 60 chrom tech your chromatography specialists agilent infinitylab poroshell 120 hplc columns 2 m frit, for rugged performance with dirty samples high efficiency and high resolution up to 50% less back pressure than sub 2 m particle compatible with 400600 bar lcs, as well as uhplcs poroshell 120 particle. View online or download agilent zorbax sbphenyl user manual. Eclipse plus phenyl hexyl columns are designed for superior peak shape with basic compounds, and deliver high efficiency and excellent peak shape with all sample types. Zorbax sbc8 pn 883975906 zorbax eclipse xdbc8 pn 993967906. From simple analysis to complex method development zorbax. Eclipse plus phenylhexyl columns are designed for superior peak shape with basic compounds, and deliver high efficiency and excellent peak shape with all sample types. Chromatographic separation was achieved on a zorbax sb phenyl column50.
Sbphenyl, zorbax sbcn, zorbax sbaq a proprietary sta tionary phase used in high aqueous mobile phases, and zorbax. To find the columns that are most similar to the one selected above, sort the column database below by f s so that the smallest f s values are listed first. Agilent zorbax eclipse xdb columns c18, c8, phenyl and cn provide four bonded phase choices for method development optimization. Zorbax eclipse pah zorbax eclipse amino acid analysis aaa zorbax carbohydrate analysis. Hplc column zorbax sbc18, 80a, 1,8 m, 3 x 50 mm, 600 bar, spl. Equivalent columns separation methods technologies inc. Zorbax phenyl phases stablebond sbphenyl, eclipse xdbphenyl and eclipse plus. Zorbax sb phenyl xbridge phenyl amitriptyline n usp 9300 t usp 1. Agilent zorbax stablebond columns use patented, unique, nonfunctional silanes with bulky disobutyl sbc18 or disopropyl sbc8, sbc3, sbphenyl.
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